Dan Buckley

Many aspects of soil microbial communities are still a mystery and soil remains a fertile habitat for discovery. My research makes use of genomic tools to examine the microbial communities that live in soils, to explore their diversity, and to delineate the mechanisms by which they influence environmental processes in terrestrial ecosystems.

Selected Journal Publications

• Emmett, B. D., Youngblut, N. D., Buckley, D. H., & Drinkwater, L. E. (2017). Plant phylogeny and life history shape rhizosphere bacterial microbiome of summer annuals in an agricultural field. Frontiers in Microbiology. 8:1-16.
• Gaby, J. C., & Buckley, D. H. (2017). The Use of Degenerate Primers in qPCR Analysis of Functional Genes Can Cause Dramatic Quantification Bias as Revealed by Investigation of nifH Primer Performance. Microbial Ecology. 74:701-708.
• Wilbanks, E. G., Salman-Carvalho, V., Jaekel, U., Humphrey, P. T., Eisen, J. A., Buckley, D. H., & Zinder, S. H. (2017). The Green Berry Consortia of the Sippewissett Salt Marsh: Millimeter-Sized Aggregates of Diazotrophic Unicellular Cyanobacteria. Frontiers in Microbiology. 8:1-12.
• Pepe-Ranney, C., Koechli, C., Potrafka, R., Andam, C., Eggleston, E., Garcia-Pichel, F., & Buckley, D. H. (2016). Non-cyanobacterial diazotrophs mediate dinitrogen fixation in biological soil crusts during early crust formation. The ISME Journal: Multidisciplinary Journal of Microbial Ecology. 10:287-298.
• Pepe-Ranney, C., Campbell, A. N., Koechli, C. N., Berthrong, S., & Buckley, D. H. (2016). Unearthing the Ecology of Soil Microorganisms Using a High Resolution DNA-SIP Approach to Explore Cellulose and Xylose Metabolism in Soil. Frontiers in Microbiology. 2016:703.
• Andam, C. P., Doroghazi, J. R., Campbell, A. N., Kelly, P. J., Choudoir, M. J., & Buckley, D. H. (2016). A Latitudinal Diversity Gradient in Terrestrial Bacteria of the Genus Streptomyces. mBio. 7:e02200-15 .
• Whitman, T., Pepe-Ranney, C., Enders, A., Koechli, C., Campbell, A., Buckley, D. H., & Lehmann, J. (2016). Dynamics of microbial community composition and soil organic carbon mineralization in soil following addition of pyrogenic and fresh organic matter. The ISME Journal: Multidisciplinary Journal of Microbial Ecology. 10:2918-2930.
• Andam, C. P., Choudoir, M. J., Nguyen, A. V., Park, H. S., & Buckley, D. H. (2016). Contributions of ancestral inter-species recombination to the genetic diversity of extant Streptomyces lineages. The ISME Journal: Multidisciplinary Journal of Microbial Ecology. 10:1731-1741.
• Gaby, J. C., & Buckley, D. H. (2014). A comprehensive aligned nifH gene database: a multi-purpose tool for studies of nitrogen-fixing bacteria. Database. bauoo1.
• Youngblut, N. D., & Buckley, D. H. (2014). Intra-genomic variation in G_+_C content and its implications for DNA stable isotope probing. Environmental Microbiology Reports.

• BIOG 2990: Introduction to Research Methods in Biology
• PLSCS/BIOMI 1120: Microbes, the Earth, and Everything
• BIOMI 2900: General Microbiology Lecture