In the study, “Gut Bacteria Are Essential for Normal Cuticle Development in Herbivorous Turtle Ants,” which published Jan. 29 in Nature Communications, the researchers specifically broke down which components the bacteria contributed so their host could build a thicker armor.
When the researchers killed off the gut microbes in plant-eating turtle ants, their exoskeletons were only half as thick as normal turtle ants. The results reveal diverse roles that gut bacteria can play in organisms.
Such findings could possibly offer new directions for biomimicry, using an example from nature to design armor or coatings, with the help of microbes.
“This is the first study to show which specific cuticle components come from nutrients provided by symbiotic bacteria,” said senior author Corrie Moreau, the Martha N. and John C. Moser Professor of Arthropod Biosystematics and Biodiversity and director and head curator of the Cornell University Insect Collection in the College of Agriculture and Life Sciences.
Diverse ant species include herbivores (plant-eaters), generalists and predators. Herbivores lack nitrogen in their diets for building some amino acids, but through evolution, symbiotic gut microbes process plant matter in the insect’s digestive system and convert it into otherwise unavailable essential amino acids. Predators and generalists acquire these building blocks for cells directly from their diets.
A handful of previous studies on beetles had shown that when gut bacteria were killed off with antibiotics, the beetle’s cuticles (exoskeleton) became thinner and paler.
In this study, Moreau and colleagues separated colonies of turtle ants and split them in half. One half, a control group, was left alone; in the other half, the gut bacteria were mostly eliminated with antibiotics.
The researchers then fed both groups foods enriched with urea, a compound with high nitrogen content. They used a nitrogen isotope in the urea so they could locate where the element might be integrated within the ants. The researchers could then track how much nitrogen the bacteria-free ants were processing on their own, and what contributions the bacteria were making to synthesize the nitrogen.
Since adult ants have fully developed exoskeletons, the feeding regimen was followed throughout the ants’ development, including the larval and pupal stages.
In the end, the researchers measured the exoskeletons and found that cuticles in bacteria-free ants were about half as thick. Using analytic chemistry, Moreau and colleagues then searched for nitrogen isotopes within components of the cuticle. In the control group, the researchers found the isotopes within chitin (a biopolymer that is a primary component in insect exoskeletons), cuticular proteins, and in “cross-linkers,” a component of the cuticle that acts as a connecting fiber.
“The insect host, or hostess in this case, is producing some components on its own that it is then investing in the cuticle,” Moreau said, “but it is relying heavily on its gut bacteria to synthesize the components it needs to build a tough cuticle.”
Christophe Duplais, a French National Centre for Scientific Research faculty member and visiting professor of chemistry and chemical biology, is the paper’s first author. Co-authors include researchers from the University of Orléans, France; Bruker Biospin, France; Calvin University in Grand Rapids, Michigan; and University of Nantes, France.
The study was funded by the French National Research Agency and the U.S. National Science Foundation.
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